evaluation of anti- hla class i antibodies in chronic rejection of kidney transplantation

studies have shown that patients who do not produce donor specific and / or panel reactive anti-hla antibodies have a longer graft survival. the purpose of this study was to evaluate the posttransplant humoral immune response towards hla-class i antigens and the measurement of the serum creatinine levels which are used in monitoring posttransplant function of kidney. serum samples from 132 renal transplant recipients were screened for preformed anti-hla class i panel reactive antibodies (pra) by means of microlympho-cytotoxicity assay. the results revealed the presence of pra in 26 (19.7%) out of 132 transplanted patients. graft function was evaluated by measurement of serum creatinine levels which revealed the mean of 1.75 mg/dl (sd: 1.08). because of clinical significance of presence of different pra amounts (>10%, > 20% and >50% of panel reactivity) in patients, correlation with kidney function status was analyzed. the obtained data highlighted a higher presence of serum creatinine levels in pra-positive patients compared to negative patients (p<0.01). these results (and further studies for class ii, …) can be used to implement new therapeutic strategies to curtail post transplant alloantibodies production and better allografts survival.